例句與造句

1. at first, 1.67 u g per well mcab all was coated on three wells of a plate, and then 1.5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0.1 % tween-20 ), the bound phage was eluted with ph 2.2 tris-gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles . ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective . after 4 rounds of panning, 11 phage clones were selected after competitive-ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
   在本實(shí)驗(yàn)中，利用隨機(jī)12肽庫對抗豬瘟病毒(classicalswinefeverviruscsfv)糖蛋白me2的單抗a11進(jìn)行表位篩選，經(jīng)過四輪篩選以后，隨機(jī)挑取11個(gè)克隆作競爭-elisa檢測，結(jié)果表明，所挑11個(gè)克隆中，有9個(gè)克隆能對me2蛋白和a11反應(yīng)產(chǎn)生抑制作用，抑制率最高可達(dá)64；dna測序以后經(jīng)過dnastar軟件分析，發(fā)現(xiàn)它們的核心序列為anwralsl，該核心序列與豬瘟病毒e2蛋白的28-35位氨基酸t(yī)twkeysh具有同源性；夾心-elisa檢測和western-blotting試驗(yàn)均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經(jīng)間接elisa試驗(yàn)證實(shí)，也能被a11識別。
2. at first, 1.67 u g per well mcab all was coated on three wells of a plate, and then 1.5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0.1 % tween-20 ), the bound phage was eluted with ph 2.2 tris-gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles . ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective . after 4 rounds of panning, 11 phage clones were selected after competitive-ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
   在本實(shí)驗(yàn)中，利用隨機(jī)12肽庫對抗豬瘟病毒(classicalswinefeverviruscsfv)糖蛋白me2的單抗a11進(jìn)行表位篩選，經(jīng)過四輪篩選以后，隨機(jī)挑取11個(gè)克隆作競爭-elisa檢測，結(jié)果表明，所挑11個(gè)克隆中，有9個(gè)克隆能對me2蛋白和a11反應(yīng)產(chǎn)生抑制作用，抑制率最高可達(dá)64；dna測序以后經(jīng)過dnastar軟件分析，發(fā)現(xiàn)它們的核心序列為anwralsl，該核心序列與豬瘟病毒e2蛋白的28-35位氨基酸t(yī)twkeysh具有同源性；夾心-elisa檢測和western-blotting試驗(yàn)均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經(jīng)間接elisa試驗(yàn)證實(shí)，也能被a11識別。
3. It's difficult to find selective ratio in a sentence. 用selective ratio造句挺難的